Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate acetyl-coenzyme A (acetyl-CoA) producing PHB in oil bearing crops such as oil palm will be advantageous In this study three bacterial genes bktB phaB and phaC which are required for the synthesis of PHB and selectable marker gene bar for herbicide Basta resistant were Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis Plant J 35: 811–821 Kode V Mudd E A Iamtham S Day A (2005) The tobacco plastid accD gene is essential and is required for leaf development Plant J 44: 237–244

Polyester synthesis in transplastomic tobacco: significant

Tobacco still serves as a model plant for novel transgene expression systems however even 1 7% PHB in dry weight would be too low for profitability and industrial applications of transplastomic PHB plants are still far away A solution to the problem of inhibitory effects might be to use an inducible PHB gene expression system

Biodegradable plastics mainly polyhydroxybutyrate (PHB) which are traditionally produced by bacterial cells have been produced in the cells of more than 15 plant species Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate acetyl-coenzyme A (acetyl-CoA) producing PHB in oil bearing crops such as oil palm will be advantageous

Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis Plant J 35: A revised medium for rapid growth and bio assays with tobacco tissue culture Physiol Plant 15: 473 – 497 OpenUrl CrossRef ↵ Petersen K Schttler M A Karcher D Thiele W Bock R (2011) Elimination of a group II intron from a plastid gene causes a

Significant progress has been made in plastid genetic engineering in tobacco and other Solanaceae plants through the use of improved regeneration procedures and transformation vectors with efficient promoters and untranslated regions Many genes encoding for industrially important proteins and vaccines as well as genes conferring important agronomic traits have been stably integrated and

Antibody production in plants and its application Plants have proven to be production systems for many forms of antibodies In the last decade the expression and assembly of immunoglobulin (Ig) heavy and light chains into functional antibodies was first shown in transgenic tobacco These immunoglobulins include full-sized IgG and IgA chimeric

A Guide to Choosing Vectors for Transformation of the

Plastid transformation originally developed in tobacco ( Nicotiana tabacum ) has recently been extended to a number of crop species enabling in vivo probing of plastid function and biotechnological applications In this article we report new plastid vectors that enable insertion of transgenes in the inverted repeat region of the plastome between the trnV and 3 ′ rps12 or trnI and trnA genes

Biodegradable plastics mainly polyhydroxybutyrate (PHB) which are traditionally produced by bacterial cells have been produced in the cells of more than 15 plant species Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate acetyl-coenzyme A (acetyl-CoA) producing PHB in oil bearing crops such as oil palm will be advantageous

Production of a Subunit Vaccine Candidate against Porcine Post-Weaning Diarrhea in High-Biomass Transplastomic Tobacco Production of dengue virus envelope protein domain III-based antigens in Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems Chloroplasts: state of research and practical

Disclosed are methods for transforming plastids and moving transformed plastids from one plant to another Also disclosed are transplastomic plants parts thereof and seed derived therefrom Further disclosed is a cell or protoplast of a plant or a culture thereof (e g a callus culture) wherein the cell or protoplast contains a plastid obtained from a cell of a genetically distinct plant

Antibody production in plants and its application Plants have proven to be production systems for many forms of antibodies In the last decade the expression and assembly of immunoglobulin (Ig) heavy and light chains into functional antibodies was first shown in transgenic tobacco These immunoglobulins include full-sized IgG and IgA chimeric

Section 1 provides an overview of the production process and biochemical characterization of therapeutic enzymes while Section 2 focuses upon the engineering strategies and delivery methods of therapeutic enzymes Section 3 highlights the clinical applications of approved therapeutic enzymes including aspects on their structure indications and mechanisms of action Together with information

Significant progress has been made in plastid genetic engineering in tobacco and other Solanaceae plants through the use of improved regeneration procedures and transformation vectors with efficient promoters and untranslated regions Many genes encoding for industrially important proteins and vaccines as well as genes conferring important agronomic traits have been stably integrated and

Tobacco chloroplast transformation was performed using pLD-201-mALS harboring G121A A122V P197S P197S S653I or W574L S653I G121A in Arabidopsis ALS corresponds to G95A in rice (Okuzaki et al 2007) The resultant transplastomic plants were maintained on hormone-free Murashige and Skoog (MS) medium (Figure 7B) It is concluded that the

Production by Tobacco Transplastomic Plants of

Production by Tobacco Transplastomic Plants of Recombinant Fungal and Bacterial Cell-Wall Degrading Enzymes to Be Used for Cellulosic Biomass Saccharification Longoni P(1) Leelavathi S(2) Doria E(3) Reddy VS(2) Cella R(4) Author information: (1)Dipartimento di Biologia e Biotecnologie Universit di Pavia Via Ferrata 9 27100 Pavia Italy Dipartimento de Biologie Vgtale

Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis Plant J 35:811–21 75 Klein TM Wolf ED Wu R Sanford JC 1987 High-velocity microprojectiles for delivering nucleic acids in living cells Nature 327:70–73 76 Knoblauch M Hibberd JM Gray JC Van 56 Annu Rev Plant Biol 2004 55:289-313 Downloaded from arjournals

Plastid transformation is an attractive alternative to nuclear transformation enabling manipulation of native plastid genes and the insertion of foreign genes into plastids for applications in agriculture and industrial biotechnology Transformation is achieved using dominant positive selection markers that confer resistance to antibiotics The very high copy number of plastid DNA means that a

This approach has proven to be efficacious in several clinical vaccination and tolerance induction trials as well as multiple preclinical studies for disease prevention The production of oral biopharmaceuticals in edible plant tissues could revolutionize the pharmaceutical industry by reducing the cost of production systems based on fermentation and also eliminating expensive downstream

Tobacco chloroplast transformation was performed using pLD-201-mALS harboring G121A A122V P197S P197S S653I or W574L S653I G121A in Arabidopsis ALS corresponds to G95A in rice (Okuzaki et al 2007) The resultant transplastomic plants were maintained on hormone-free Murashige and Skoog (MS) medium (Figure 7B) It is concluded that the

Many animals have evolved the capacity to produce light from chemical reactions For example an enzyme known as luciferase in fireflies produces light by acting on a molecule called luciferin Scientists have identified the enzymes that drive several of these systems and used them to build reporters that can study the activity of genes in the tissues of plants and other lifeforms over space

Nevertheless in generating transplastomic tobacco expressing a 25 kda protein antigen against Mycobacterium leprae and Mycobacterium avium Hassan et al successfully transformed the mmpI gene along with an adjuvant lymphotoxin-beta (LTB) into tobacco chloroplast by the Polyethylene glycol (PEG) mediated transformation method [74]

Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis Klaus et al The Plant Journal (2003) 35 811-821 Overexpression of the Bacillus thuringiensis (Bt) Cyr2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt -resistant insects Kota et al Proc Natl Acad Sci USA vol 96 pp 1840-1845 Mar 1999

Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis Sebastian M J Klaus ICON Genetics AG Research Centre Freising Lise‐Meitner‐Str 30 85354 Freising Germany Search for more papers by this author Fong‐Chin Huang ICON Genetics AG Research Centre Freising Lise‐Meitner‐Str 30 85354 Freising Germany Search for

Here we report two transplastomic tobacco lines with functional Rubisco from the cyanobacterium Synechococcus elongatus Rapid advances in DNA synthesis techniques have made it possible to engineer viruses biochemical pathways and assemble bacterial genomes Here we report the synthesis of a functional 272 871–base pair designer eukaryotic chromosome synIII which is based on the

01/03/2010Tobacco has also proven to be an efficient system for generating fragments of the antibody including single-chain variable fragment (scFv) single-chain antibody variable domain (Fv) fragment and antibody-binding (Fab) fragment (see Table 1) These small recombinant synthetic antibodies retain full antigen-binding activity but lack specific assembly requirements